fluorescent glucose analog 2 nbdg Search Results


90
FUJIFILM fluorescently labeled deoxy-glucose analog (2-nbdg
BCAAs promoted glucose uptake in HeLa cells. HeLa cells were cultured in an amino acid-free medium containing 4.5 g/L of glucose with or without 40 mM BCAAs ( A – I ) and with or without 7 µg/mL tunicamycin (TM; endoplasmic reticulum stress inducer) ( D - F ) or 75 mM <t>2-deoxy-D-glucose</t> (2-DG, glycolysis hexokinase inhibitor) ( G – I ) for 24 hours before addition of fluorescently labeled deoxy-glucose analog (100 µM 2-NBDG). A , B , D , E , G , H Histograms showing distribution of cell counts <t>for</t> <t>fluorescence</t> intensities (FL) by flowcytometry analysis. C , F , I Geometric average of fluorescence intensities of live cells. C *** P < 0.0001, unpaired t -test, F , I *** P < 0.005, Tukey HSD, N = 4. Bars represent the standard deviation.
Fluorescently Labeled Deoxy Glucose Analog (2 Nbdg, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+glucose+analog+2+nbdg/fluorescently+labeled+deoxy+glucose+analog++2+nbdg/pmc09363681-22-10-13
Average 90 stars, based on 1 article reviews
fluorescently labeled deoxy-glucose analog (2-nbdg - by Bioz Stars, 2026-09
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90
ApexBio fluorescence-based 2-nbdg assay kit
BCAAs promoted glucose uptake in HeLa cells. HeLa cells were cultured in an amino acid-free medium containing 4.5 g/L of glucose with or without 40 mM BCAAs ( A – I ) and with or without 7 µg/mL tunicamycin (TM; endoplasmic reticulum stress inducer) ( D - F ) or 75 mM <t>2-deoxy-D-glucose</t> (2-DG, glycolysis hexokinase inhibitor) ( G – I ) for 24 hours before addition of fluorescently labeled deoxy-glucose analog (100 µM 2-NBDG). A , B , D , E , G , H Histograms showing distribution of cell counts <t>for</t> <t>fluorescence</t> intensities (FL) by flowcytometry analysis. C , F , I Geometric average of fluorescence intensities of live cells. C *** P < 0.0001, unpaired t -test, F , I *** P < 0.005, Tukey HSD, N = 4. Bars represent the standard deviation.
Fluorescence Based 2 Nbdg Assay Kit, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+glucose+analog+2+nbdg/fluorescent+glucose+analog+2++nbdg/pmc11610135-293-6-10
Average 90 stars, based on 1 article reviews
fluorescence-based 2-nbdg assay kit - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

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BCAAs promoted glucose uptake in HeLa cells. HeLa cells were cultured in an amino acid-free medium containing 4.5 g/L of glucose with or without 40 mM BCAAs ( A – I ) and with or without 7 µg/mL tunicamycin (TM; endoplasmic reticulum stress inducer) ( D - F ) or 75 mM 2-deoxy-D-glucose (2-DG, glycolysis hexokinase inhibitor) ( G – I ) for 24 hours before addition of fluorescently labeled deoxy-glucose analog (100 µM 2-NBDG). A , B , D , E , G , H Histograms showing distribution of cell counts for fluorescence intensities (FL) by flowcytometry analysis. C , F , I Geometric average of fluorescence intensities of live cells. C *** P < 0.0001, unpaired t -test, F , I *** P < 0.005, Tukey HSD, N = 4. Bars represent the standard deviation.

Journal: Investigative Ophthalmology & Visual Science

Article Title: Branched Chain Amino Acids Promote ATP Production Via Translocation of Glucose Transporters

doi: 10.1167/iovs.63.9.7

Figure Lengend Snippet: BCAAs promoted glucose uptake in HeLa cells. HeLa cells were cultured in an amino acid-free medium containing 4.5 g/L of glucose with or without 40 mM BCAAs ( A – I ) and with or without 7 µg/mL tunicamycin (TM; endoplasmic reticulum stress inducer) ( D - F ) or 75 mM 2-deoxy-D-glucose (2-DG, glycolysis hexokinase inhibitor) ( G – I ) for 24 hours before addition of fluorescently labeled deoxy-glucose analog (100 µM 2-NBDG). A , B , D , E , G , H Histograms showing distribution of cell counts for fluorescence intensities (FL) by flowcytometry analysis. C , F , I Geometric average of fluorescence intensities of live cells. C *** P < 0.0001, unpaired t -test, F , I *** P < 0.005, Tukey HSD, N = 4. Bars represent the standard deviation.

Article Snippet: To quantify the glucose uptake, the fluorescence of fluorescently labeled deoxy-glucose analog (2-NBDG; Wako) was quantified using flow cytometry.

Techniques: Cell Culture, Labeling, Fluorescence, Standard Deviation

Inhibition of glucose transporters prevented BCAAs from promoting glucose uptake. HeLa cells were cultured in an amino acid-free medium containing 4.5 g/L of glucose with or without 40 mM BCAAs for 3 hours and then incubated with or without 250 µM WZB117 (inhibitor of glucose transporter [GLUT] 1, 3, and 4) for 1 hour before addition of fluorescently labeled deoxy-glucose analog (100 µM 2-NBDG). A – C Glucose uptake with or without BCAAs and with or without WZB117 in HeLa cells. A , B Histograms showing distributions of cell counts for fluorescence intensities (FL) by flowcytometry analysis. C Geometric average of fluorescence intensities of live cells. D Cell viability with or without BCAAs and with or without WZB117 in HeLa cells. C , D *** P < 0.005, N.S. no significant difference, Tukey HSD, N = 4 each. Bars represent standard deviation.

Journal: Investigative Ophthalmology & Visual Science

Article Title: Branched Chain Amino Acids Promote ATP Production Via Translocation of Glucose Transporters

doi: 10.1167/iovs.63.9.7

Figure Lengend Snippet: Inhibition of glucose transporters prevented BCAAs from promoting glucose uptake. HeLa cells were cultured in an amino acid-free medium containing 4.5 g/L of glucose with or without 40 mM BCAAs for 3 hours and then incubated with or without 250 µM WZB117 (inhibitor of glucose transporter [GLUT] 1, 3, and 4) for 1 hour before addition of fluorescently labeled deoxy-glucose analog (100 µM 2-NBDG). A – C Glucose uptake with or without BCAAs and with or without WZB117 in HeLa cells. A , B Histograms showing distributions of cell counts for fluorescence intensities (FL) by flowcytometry analysis. C Geometric average of fluorescence intensities of live cells. D Cell viability with or without BCAAs and with or without WZB117 in HeLa cells. C , D *** P < 0.005, N.S. no significant difference, Tukey HSD, N = 4 each. Bars represent standard deviation.

Article Snippet: To quantify the glucose uptake, the fluorescence of fluorescently labeled deoxy-glucose analog (2-NBDG; Wako) was quantified using flow cytometry.

Techniques: Inhibition, Cell Culture, Incubation, Labeling, Fluorescence, Standard Deviation